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KMID : 1189420170300040037
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2017 Volume.30 No. 4 p.37 ~ p.48
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A Study of Melanin Synthesis Pathway of the Ethanol extract of Eclipta prostrata In Vitro Study
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Park In-Hae
Hong Seok-Hoon
Woo Won-Hong
Mun Yeun-Ja
Cha Su-Bin
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Abstract
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Objectives : This Study was conducted to investigate the melanin synthesis effect of Eclipta prostrata and to determine the relationship between melanin synthesis effect of Eclipta prostrata and cAMP/PKA melanin synthesis pathway.
Methods : We measured melanin contents, tyrosinase activity, expression of TRPs, p-CREB in B16F10 cells cultured with Eclipta prostrata ethanol extracts(EEP). And after treatment with H89 and dibutyryl cAMP, which inhibit or promote the activation of PKA, we observed changes in melanin synthesis and tyrosinase activity stimulated by EEP.
Results : EEP increased melanin synthesis by promoting the expression of tyrosinase and TRP-1. It also promoted expression of p-CREB. H89 suppresses melanogenic effect and expression of tyrosinase, TRP-1 in B16F10 stimulated by EEP. Dibutyryl cAMP promotes melanogenic effect of EEP.
Conclusions : The results of this study suggest that melanin synthesis effect of EEP is related to induction of tyrosinase and TRP-1 expression through the cAMP/PKA pathway.
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KEYWORD
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Eclipta prostrata , melanin
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